T-bet: a bridge between innate and adaptive immunity Originally described over a decade ago as a T cell transcription factor regulating T helper 1 cell lineage commitment, T-bet is now recognized as having an important role in many cells of the adaptive and innate immune system From Wikipedia, the free encyclopedia T-box transcription factor TBX21, also called T-bet (T-box expressed in T cells) is a protein that in humans is encoded by the TBX21 gene T-bet, a T-box transcription factor, is expressed in CD4+ T lymphocytes committed to Th1 T-cell development and may participate in immunoglobulin class switching in B lymphocytes. T-bet is also expressed in a subset of T-cell lymphomas, particularly those that express other markers of Th1 T cell dif T-bet (encoded by Tbx21) is an immune cell-specific member of the T-box family of transcription factors (Fig. 1). The adaptive immune system arose approximately 500 million years ago in jawed fish..
Anti-T-bet Antikörper 4B10 ist ein monoklonales IgG 1 (kappa light chain) T-bet Antikörper in einer Menge von 200 µg/ml raised against full length recombinant murine T-bet Anti-T-bet Antikörper (4B10) ist empfohlen für die Detektion von T-bet aus der Spezies mouse, rat und human per WB, IP, IF, FCM und ELIS T-bet is a 530-amino acid protein with a 189-amino acid T box DNA-binding domain (underlined). (B) T-bet shares a region of homology with the T box family members T-brain and eomesodermin. Bold lettering indicates shared amino acid identity Main Text. Differential expression of the transcription factor T-bet (encoded by the gene Tbx21) is known to delineate functionally distinct T cell subsets.Certain memory B cells (MBCs) also express T-bet, although their core properties and relationship to T-bet − MBCs remain unclear. T-bet + MBCs have been best characterized in the context of dysregulated immune responses such as systemic. T-bet, also known as T-box transcription factor T-bet, is considered to be a master regulator of Th1 lymphoid development controlling the production of the cytokine IFN-γ. T-bet is widely expressed in hematopoietic cells including stem cells, NK cells, B cells, and T cells
Herzlich willkommen bei T-Beton Wir sind die Experten für Transportbeton und Betonpumpenvermietung. Wir beliefern Kunden in und um Bösel und bieten für jeden Einsatzzweck die richtige Betonsorte: vom klassischen Fertigbeton bis zum modernen Transportbeton, der sich je nach Bedarf leicht verarbeiten lässt T-bet, also known as TBX21, is a T-cell specific transcription factor containing a single T-box DNA-binding domain. It controls the.. T-bet, also known as TBX21, is a T-cell specific transcription factor containing a single T-box DNA-binding domain. It controls the expression of the TH1 cytokine, interferon-gamma. Both Th1 and Th17 cells are crucial in immune regulation and autoimmune disease development
In Th1 cells, T-bet associates with genes of diverse function, including those with roles in transcriptional regulation, chemotaxis and adhesion. GATA-3 occupies genes in both Th1 and Th2 cells and, unexpectedly, shares a large proportion of targets with T-bet. Re-complementation of T-bet alters the expression of these genes in a manner that mirrors their differential expression between Th1 and Th2 lineages. These data show that the choice between Th1 and Th2 lineage commitment is the result. BD Pharmingen™ PE Mouse anti-T-Bet. Clone: 4B10 Size: 50 Tests Status: RUO Cat No.: 561265. Quick View. $237.00. Product Results 1 - 14 of 14. Showing 20 50 100 item per page
T-bet, also as known as TBX21, plays a critical role in development and maintenance of type 1 helper T (Th1) and T-bet deficient mice display impaired Th1 differentiation (10,11). Wilkinson, D.G. et al. (1990) Nature 343, 657-9. Papaioannou, V.E. and Silver, L.M. (1998) Bioessays 20, 9-19. Showell, C. et al. (2004) Dev Dyn 229, 201-18. Papaioannou, V.E. (2001) Int Rev Cytol 207, 1-70. Hoogaars. This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is the human ortholog of mouse Tbx21/Tbet gene .p. and killed 14 days later. Spleen cells were then cultured with either the LCMV MHC-I peptide, GP33, or plate-bound anti-CD3 and anti-CD28, and intracellular IFN-γ production was determined by FACS. Secondary CTL assays were performed 30 days after LCMV infection. Spleen. A detection system for GATA-3 und T-Bet was already established within the working group for the analysis of these factors in total protein fractions from cell lines using polyclonal antibodies. In this work, that system was successfully adapted for the analysis of other sample materials
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TGFβ2 an der Entwicklung von Lunge, Herz, Knochen und Urogenitaltrakt beteiligt ist, TGFβ3 ebenfalls zur Lungenentwicklung beiträgt. TGFβ3 Signale sind außerdem wichtig um die Entstehung von Gaumenspalten zu verhindern. Alle drei TGFβ scheinen in der Lage, Zellen verschiedenen Ursprungs in Myofibroblasten zu transformieren T‐bet and Eomes regulation and role in anti‐tumor responses. Tumor immune‐surveillance is severely impaired in Tbx21 −/ − mice, as firstly shown in the transgenic adenocarcinoma mouse prostate model 123. Although the absence of T‐bet did not impact the response against the primary tumor, T‐bet was required to suppress metastasis development. This indicates that T‐bet regulates. T-bet Antibody (4B10) is available as both the non-conjugated anti-T-bet antibody form, as well as multiple conjugated forms of anti-T-bet antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor ® conjugates. T helper (Th) lymphocytes differentiate into two unique subsets, Th1 and Th2, which differ both in function and in the cytokines they secrete. Th1 and Th2 cytokines promote. . Zet direct in op Voetbal en vele andere Sporten
T-bet is expressed in a significant subset of B-cell lymphoproliferative disorders, particularly at an early stage of B-cell development (precursor B-cell lymphoblastic leukemia/lymphoblastic lymphoma), and B-cell neoplasms derived from mature B-cells, including CLL/SLL, marginal zone lymphoma, and hairy cell leukemia. In contrast, B-cell neoplasms derived from pre-germinal center or germinal. The identification of T-bet as a key transcription factor associated with the development of IFNγ-producing CD4+ T cells predicted a crucial role for T-bet in cell-mediated immunity and in. T-bet is a member of the T-box family of transcription factors that appears to regulate lineage commitment in CD4 T helper (TH) lymphocytes in part by activating the hallmark TH1 cytokine, interferon-γ (IFN-γ). IFN-γ is also produced by natural killer (NK) cells and most prominently by CD8 cytotoxic T cells, and is vital for the control of microbial pathogens Multiple T-bet functions playing a role in Th cell differentiation. Induction of T-bet expression through activation of STAT1 and STAT4 directly stimulates the transcription of T-box-binding element-containing genes, such as IFNG, IL12RB2, and CXCR3, thereby enhancing Th1 cell development.T-Bet undergoes serine phosphorylation at S508 and then downregulates IL-2 production in Th1 cells by.
T-bet is required for optimal production of IFN-gamma and antigen-specific T cell activation by dendritic cells. Proc Natl Acad Sci U S A. 2003; 100(13):7749-7754. View reference. Peng SL, Szabo SJ, Glimcher LH. T-bet regulates IgG class switching and pathogenic autoantibody production. Proc Natl Acad Sci U S A. 2002; 99(8):5545-5550. View reference. Peng SL. The T-box transcription factor T. T-Bet and Eomes Regulate the Balance between the Effector/Central Memory T Cells versus Memory Stem Like T Cells Gang Li1., Qianting Yang2,3., Yibei Zhu2,4., Hong-Rui Wang5, Xinchun Chen3, Xueguang Zhang1, Binfeng Lu2* 1Stem Cell Research Laboratory of Jiangsu Province, Jiangsu Institute of Clinical Immunology, Institute of Medical Biotechnology, Soochow University, Suzhou, Jiangsu Human T-bet is 535 amino acids in length and contains a T-box DNA binding domain (aa 136-327). Human T-bet shares 88% aa sequence identity with mouse T-bet. T-bet is a nuclear protein highly apparent in Th1-cells. Northern blot analysis revealed that it is also expressed in lung, thymus and spleen. Functionally, T-bet controls the expression of the Th1 cytokine, IFN gamma, and initiates Th1.
T-bet-dependent expression of osteopontin in T cells is essential for efficient skewing of CD4+ T and CD8+ T cells toward the type 1 T helper and type 1 CD8+ T cells (Tc1) pathway, respectively. In T-bet-deficient mice that spontaneously develop asthma-like symptoms, the airways provide a fertile environment that promotes colonization by Mycoplasma spp. and is ill-equipped to kill and clear. This website is operated by Arena Sports Entertainment N.V. Ltd and licensed by the Curacao Chamber of Commerce and Industry Reg.# 8048/JAZ2020-041 - issue date 15/07/202
Also Known As:T-bet-. T cells from the lymph nodes of T-bet-mice lack T-box transcription factor TBX21, and may be useful in studies of acute and chronic asthma and chronic intestinal inflammation.. Donating Investigato T-bet gene might be involved in the development of NK/T-cell lymphoma. distribution of T-BET/TBX21 expression in the haematopoietic compartment; A key transcription factor that links the long-term renewal of memory CD8+ T cells to their characteristic effector potency. Among twenty-three novel polymorphisms identified in the human TBX21 gene of Korean asthma patients, no significant. Here, we found that T-bet was specifically expressed in Type I TFH cells but not Type II TFH cells. While dispensable for the early fate commitment of TFH cells, T-bet was essential for the maintenance of differentiated TFH cells, promoting their proliferation, and inhibiting their apoptosis during acute viral infection. Microarray analysis showed both similarities and differences in. Innate lymphoid cells (ILC) play a significant immunological role at mucosal surfaces such as the intestine. T-bet-expressing group 1 innate lymphoid cells (ILC1) are believed to play a substantial role in inflammatory bowel disease (IBD). However, a role of T-bet-negative ILC3 in driving colitis has also been suggested in mouse models questioning T-bet as a critical factor for IBD Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human T cell lymphoma tissue labelling T-bet / Tbx21 with unpurified ab150440 at 1/250 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody [EPR9301] (ab150440.
. SLE × T-bet fl/fl littermates were used as controls for all experiments. Blood collection and proteinuria assessment were. Anti-T-bet / Tbx21 antibody (ab154058) at 1/3000 dilution + NT2D1 whole cell lysate at 30 µg Predicted band size: 58 kDa 7.5% SDS PAGE . Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-T-bet / Tbx21 antibody (ab154058) Immunohistochemical analysis of paraffin-embedded DLD-1 xenograft tissue labeling T-bet / Tbx21 with ab154058 at 1/100 dilution. Protocols. Western. T-bet and Zeb2 have been shown to be critical for terminal NK cell maturation (Townsend et al., 2004; van Helden et al., 2015). The signaling downstream of IL-15 receptors are essential for regulating the expression of these transcription factors. PDK1 is required for the induction of E4BP4 during early NK cell development.
Human T-bet is 535 amino acids in length and contains a T-box DNA binding domain (aa 136-327). Human T-bet shares 88% aa sequence identity with mouse T-bet. T-bet is a nuclear protein highly apparent in Th1 cells. Northern blot analysis revealed that it is also expressed in lung, thymus and spleen. Functionally, T-bet controls the expression of the Th1 cytokine, IFN-gamma, and initiates Th1. Zuerst der Mensch! Das ist seit über 25 Jahren das Motto für unser erfolgreiches Direktmarketing der prowin B&T. Reinigungsmittel, Kosmetik und Tiernahrung T-bet and Eomes represent two key transcription factors for CD8+ T cell differentiation and function, but surprisingly little is known about their influence of effector immunity following chronic viral infections in humans. In this study, we demonstrate that HIV-specific CD8+ T cells possess highly elevated levels of Eomes, but low T-bet expression. This differential relationship is linked to. T-bet plays a central role in this balance, acting both intrinsically in GC B cells to promote IgG2a/c and indirectly, presumably through TFH cytokine production, to lower IgG1 production. Combined, these results identify T-bet as a context-dependent link between extrinsic inflammatory signals and intrinsic cellular differentiation programs and highlights the importance of understanding the. How cytokines control differentiation of helper T (TH) cells is controversial. We show that T-bet, without apparent assistance from interleukin 12 (IL-12)/STAT4, specifies TH1 effector fate by targeting chromatin remodeling to individual interferon-γ (IFN-γ) alleles and by inducing IL-12 receptor β2 expression. Subsequently, it appears that IL-12/STAT4 serves two essential functions in the.
I wouldn't bet on it phrase. What does I wouldn't bet on it expression mean? Definitions by the largest Idiom Dictionary. What does I wouldn't bet on it expression mean? Definitions by the largest Idiom Dictionary T-bet expression peaked at day 8 p.i. in Th1 cells, gradually declining by day 15. T-bet expression also decreased in Tfh cells by day 10 p.i, with the remaining transcription factor-positive Tfh cells expressing Bcl6. These observations were not specific to TCR transgenic T cells, as the endogenous Tfh cell population had similar kinetics to. Online betting company. Bet on Premier League, Champions League and International Football, Tennis. Watch Visualization Live Sport Don't Bet Money Honey is a song written and performed by Linda Scott. It reached #3 on the US adult contemporary chart, #9 on the Billboard Hot 100, and #50 on the UK Singles Chart in 1961.. The song was produced and arranged by Hutch Davie.. The single ranked #36 on Billboard's Year-End Hot 100 singles of 1961.. The B-side to the single, Starlight, Starbright, reached #44 on the Billboard.
Willkommen bei der FIMBAG, der Finanzmarktbeteiligung Aktiengesellschaft des Bundes. Wir haben die Kapitalmaßnahmen der Republik Österreich nach dem Finanzmarktstabilitätsgesetz (FinStaG) durch Übernahme und Verwaltung von Partizipationskapital oder sonstigen Kapitalbeteiligungen des Bundes an Banken als Treuhänder für die Republik Österreich unterstützt Lineage-defining transcription factor which initiates Th1 lineage development from naive Th precursor cells both by activating Th1 genetic programs and by repressing the opposing Th2 and Th17 genetic programs. Activates transcription of a set of genes important for Th1 cell function, including those encoding IFN-gamma and the chemokine receptor CXCR3 The results suggested that siRNA‑mediated knockdown of T‑bet regulated the immune balance of Th17/Tregs via changes to RORγt and FOXP3. When siRNA against RORγt and T‑bet were used in combination, a stronger ability to regulate immune balance was observed. These results imply that Th1‑ and Th17‑type immunity is dominant in PE and. Without T-bet, the mix of intestinal bacteria appears to change, favoring dangerous pathogens, the investigators observed. A big surprise was that T-bet knockout mice in which ulcerative colitis developed passed the disease to offspring with normal T-bet levels, and even to unrelated healthy adult mice, presumably through oral-fecal contact. The researchers didn't know which types of. Definition of don't bet on it in the Idioms Dictionary. don't bet on it phrase. What does don't bet on it expression mean? Definitions by the largest Idiom Dictionary. What does don't bet on it expression mean
UK National Lottery Hacked: You didn't Bet on this! Posted on March 18, 2018 at 7:52 AM UK National Lottery Hacked: You didn't Bet on this! Some ten million lottery customers in the UK are being advised to update their credentials. The National Lottery site was attacked last week by cybercriminals. Around 150 accounts were compromised, and fewer than ten were accessed for activity. We all want EVs that can charge fully in five minutes. Recently, a company said it had cracked that, but the reality is that the tech is quite far away C'mon Baby Don't Bet Against Me Songtext von The Hackensaw Boys mit Lyrics, deutscher Übersetzung, Musik-Videos und Liedtexten kostenlos auf Songtexte.co Don't Bet on Love ein Film mit Lew Ayres, Ginger Rogers. Inhaltsangabe: Der Klempner Bill (Lew Ayres) gewinnt eine große Summe Geld an der Rennbahn, gerät dann aber in Schwierigkeiten. Nur seine. Don't bet on the silver boom. The industrial metal will not be dragged up by gold. John Dizard Add to myFT. There are billions of ounces of silver that has already been mined and then stored as.
By 2050, China will be the world's largest economy, followed by India, and the U.S. in third place. That's according to a recent PwC report, which predicts that China will ac count for 20 percent. One stock that might be an intriguing choice for investors right now is Cognex Corporation CGNX. This is because this security in the Electronics - Testing Equipment space is seeing solid earnings. Tesla stock is still an EV darling. It's just that markets are wondering aloud about its price. I wouldn't bet against it. More From InvestorPlace Why Everyone Is Investing in 5G All WRONG Top. Absence of T-bet and ROR[gamma]t does not alter donor antitumor (p815) response. Lethally irradiated (1200 cGy) B6D2F1 mice were transplanted with grafts from WT or RORγt −/ − /T-bet −/− donors, containing 5 × 10 6 TCD-BM cells and 2-4 × 10 6 T cells from WT, T-bet −/− /RORγt −/− donors. No (A) or 4 × 10 3 P815 tumor cells (B-C) were added into donor graft. Survival by.
I wouldn't bet on it. There wasn't any room to fit it into the narrative and the story I was telling in the performance. So yeah, there's no special guests. And the 30-year-old singer has. 7 Followers, 6 Following, 0 Posts - See Instagram photos and videos from CENTI G. (@gtbettulsais This is not news to people in the industry — but it is to the public. Most people don't realize that even if personal information is stripped away or is not collected directly, it's often.
T-bet is the key transcription factor involved in the regulation of Th1 development and function. DP1 deficiency resulted in remarkable increase in T-bet protein expression in T cells, without affecting its mRNA expression (Figure 3A and 3B) By using another T-bet fate-mapping mouse strain, T-bet-Cre-tdTomato (Fig. S2, C and D), in which all cells that are currently expressing or have previously expressed T-bet are labeled by tdTomato expression in the absence of TMX, we confirmed that all the cells capable of IFN-γ production were within the population that had expressed T-bet (Fig. 2 C and Fig. S2 E). To further confirm IFN-γ production without in vitro restimulation and to visualize IFN-γ-expressing ex-T-bet Tfh.
T-bet expression was characterized by immunoblotting with anti-T-bet 4B10 monoclonal Ab and anti-Flag Ab. Anti-GAPDH antibody was used as an endogenous control. (G) Mutations of individual residues in the T-bet cDNA (E156S, M157L, E156A, and E157A) were generated in pCMV6 plasmids by site-directed mutagenesis. The plasmids were used to transfect HEK293T cells for overexpression. T-bet levels were measured by immunoblotting with anti-T-bet 4B10 Ab and anti-Flag Ab. Anti-GAPDH Ab was used as. Der Transforming Growth Factor ist ein zu den Zytokinen zählendes Signalmolekül. Er ist namensgebend für die TGF-Signalwege. TGF spielen eine sehr wichtige Rolle bei der embryonalen Entwicklung und der Differenzierung von Zellen und Geweben. Sie werden in verschiedene Gruppen eingeteilt: in die TGFα sowie die TGFβ. Beide Gruppen von Zytokinen sind weder strukturell miteinander verwandt, noch nutzen sie ähnliche Signalwege. Die Benennung hat historische Gründe We found that after acute infection with lymphocytic choriomeningitis virus (LCMV), T-bet was coexpressed with Bcl6 in Tfh cells, with the former transcription factor necessary for their expansion and secretion of IFN-γ and with a correspondingly robust GC output. T-bet expression in Tfh cells was STAT4 dependent, as it is in Th1 cells. Although production of IL-21 was independent of T-bet expression, it was dependent on STAT4 signaling. Thus, Tfh cells, like their Th1 counterparts. Although T-bet was not required for the development of THGM cells, it was essential for their encephalitogenicity. These findings demonstrate that THGM cells constitute a distinct population of TH cells with lineage characteristics that are poised to adopt a TH1 phenotype and promote neuroinflammation. Production of GM-CSF by T helper cells not only guides adaptive immune responses but also.
Definition of I wouldn't bet on it. —said to indicate the listener's disagreement with a statement The economy will improve next year. I wouldn't bet on it if I were you Ethereum Just Shot Up $250 But Don't Bet on That Resistance Breaking. Nick Chong | Jun 24, 2020 | 10:00 . Share. Tweet. Send. Share. While Bitcoin was rejected at the $9,800 resistance, Ethereum has continued to press higher. The second-largest cryptocurrency is up by around 4% from the past 24 hours' lows, outpacing Bitcoin's relatively mild 1-2% gain. ETH's latest uptick brings it. Directed by Murray Roth. With Lew Ayres, Ginger Rogers, Charley Grapewin, Shirley Grey. Bill McCaffery, a plumber, wins big at the racetrack but then his luck runs out and almost ruins his business. Molly Gilbert, his manicurist girlfriend, stands by him and helps him readjust to life as a plumber Don't bet on a return to normalcy. by Jonathan Tobin | November 05, 2020 11:00 PM Print this article. F or those who longed for a respite from the 24/7 political and culture wars of the past four.
AN expert hinted Brits can't bet on EU summer holidays today - warning we must keep the South African variant out of the country. Professor Neil Ferguson said important decisions are The national election campaign in the Netherlands has been dominated by Covid-19, not EU membership, writes David Harding. Wednesday 17 March 2021 00:01. comments You create data every time you push the 'Like' button on Facebook, search for something on Google, make a purchase on Amazon, watch a show on Netflix, send a text on your mobile phone or do a. Entertainment Music International. Recording artist The Weeknd speaks at the halftime show press conference ahead of the Super Bowl 55 football game, Thursday, Feb. 4, 2021, in Tampa, Fla. Picture. T-bet is a member of the Tbox family of transcription factors; however, T-bet coordinately regulates the expression of many more genes than other Tbox proteins. A central unresolved question is how T-bet is able to simultaneously recognize distant Tbox binding sites, which may be located thousands of base pairs away. We have determined the crystal structure of the Tbox DNA binding domain (DBD.
don't bet on it / I wouldn't bet on it phrase. I'd lay money / odds / a bet on something phrase. you can bet your life / your bottom dollar phrase. Word Forms +-present tense: I/you/we/they: bet: he/she/it: bets: present participle: betting: past tense: bet: past participle: bet: DEFINITIONS 1. 1. to risk an amount of money by saying what you think will happen, especially in a race or. Don't bet on 2021: Remember how we dumped 2019 so fast at midnight and look what that did to us December 7, 2020, 9:56 PM IST Bikram Vohra in TOI Edit Page , Edit Page , Humour , India , TO UK Politics Odds: Don't bet on Rishi Sunak's popularity lasting. Max Liu; 07 February 2021; 5:00 min read; Rishi Sunak has had a whirlwind year as chancellor. Join today. View market. Twitter. Super Bowl Props to Bet With Friends Who Don't Bet. Super Bowl 55 Promos: Get Over $1,500 From These No-Brainer Offers! Read now. Over/Under on the National Anthem. One of the most controversial and fun bets you can make on the Super Bowl takes place before the ball is even kicked off. Whether you bet $1, $10 or $1000 there will be no differentiation in the sweat trickling down anyone's. T-bet is essential to the development of T lymphocytes, directing the differentiation of Th0 cells into Th1 or Th2 cells (1,6,20). Numerous studies demonstrate that silencing T-bet may have an apparent effect on autoreactive T cells and autoimmunity (21 -23). The RNA interference technique has become a convenient and easy way to knock down target genes (24-26). We used the RNA interference.
Don't Bet On It. Credit... Hannah K. Lee. By Mikkael A. Sekeres, M.d. and Timothy D. Gilligan, M.d. March 1, 2017; We want to let you in on a secret. As your oncologists, we'd like to treat. Don't bet against a third wave, third COVID lockdown in Ontario Post navigation < > Science advisory panel sees warning signs. By Dan Blakeley Published: Feb 12th, 2021 9:21am Last Updated: Feb 12th, 2021 9:36am. Facebook Twitter. Local health officials and Barrie Mayor Jeff Lehman are not the only ones worried the reopening of the provincial economy may be too much, too soon. Ontario's. Hier sollte eine Beschreibung angezeigt werden, diese Seite lässt dies jedoch nicht zu Why the NCAA wants to trademark 'DON'T BET ON IT' The NCAA has typically used the phrase in consumer education programs during its annual basketball championship tournaments. The thrust of the campaign is to discourage people from placing bets on the games. That's because the NCAA and its members fear that gambling interests could compromise the integrity of the contests. While some.
I bet definition: 1. said to show that you understand why someone has a particular opinion or feels a particular way. Learn more